Mickey Mouse in Action is a coming up with animation and live-action movie. It's like Who Framed Roger Rabbit? (1988 film), Space Jam (1996), The Adventures of Rocky and Bullwinkle (2000) and Looney Tunes Back in Action (2003).
- Kieran Glen Harris Stark (in Mickey Mouse voice) as Mickey Mouse
- Cindy Robinson (in Amy Rose voice) as Minnie Mouse
- Mitchell Kuester (in Donald Duck voice) as Donald Duck
- Kieran Glen Harris Stark (in Daisy Duck voice) as Daisy Duck
- Glen Campbell (in Goofy voice) as Goofy
- Roger Craig Smith (in Pluto voice) as Goofy
- Bob Brians (in Peg-Leg Pete voice) as Peg-Leg Pete
Actors in Live-Action
- Ewan McGregor as a man who can help Mickey, Minnie, Donald, Daisy, Goofy and Pluto to save the world.
- Harold Ramis as a villain who worked with Pete
- Piper Perabo as a woman who also helped Mickey, his friends and a man she falls in love with.
- Raffey Cassidy as a girl who lost her father because he was kidnapped by those villains so Mickey and his friends must help her find her father.
- Bill Pullman as a girl's father who got kidnapped by those villains.
- Eddie Murphy
- Emma Stone
- Alec Baldwin
- Chevy Chase
- Holly Hunter
- Bill Murray